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신경발생학실험실

신경발생학실험실
Yun Kee (기윤) 교수님
Yun Kee (기윤) 교수님
  • 분야 : 신경발생학 및 배아발생학
  • 교수 오피스 : 의생명과학관 B동 205호
  • 연구실 : 의생명과학관 B동 201호, 217호
실험실 소개
  • Embryology, Imaging, Genetics & Genomics, Bioinformatics를 통합하여 제브라피쉬 배아 발생을 관장하는 유전자의 기능, 작용기전 및 유전자 발현조절 네트웍 규명.
  • 제브라피쉬 질병동물 모델 개발 및 질병기전 연구.
  • 제브라피쉬 배아발생 모델을 이용한 향장 및 독성 테스트 등 산업적 응용기술 개발.
연구주제
  • TALEN과 CRISPR/RGEN 등의 genome engineering 기술을 이용하여 배아조직 발생 유전자들의 기능 및 작용 기전 연구
  • 제브라피쉬 배아발생 연구를 위한 나노바디 및 항체 이용 단백질분해 기술개발과 응용
  • Light-sheet microscopy, confocal microscopy를 이용한 제브라피쉬 배아발생 이미징
  • 제브라피쉬 배아발생에서 뇌구조 및 활성 연구를 통해 neural circuit 규명
  • 제브라피쉬 배아발생을 모델로 줄기세포 및 암세포 증식, 전이의 기전 연구
  • 제브라피쉬 질병동물 모델 구축과 치료제 발굴 위한 스크린
  • 제브라피쉬를 이용한 환경물질 독성 테스트
  • 제브라피쉬를 이용한 향장재료 개발
연구원
Kee lab members
Name Status email
Ahmed A. Raslan 박사과정 ahmed.raslan8080@gmail.com
곽진아 석사과정 wlswns02@naver.com
정유정 석사과정 joopn@naver.com
Ashraf U. Chowdhury 석사과정 ashraf.rx@gmail.com
김단비 석사과정 kdb0515@naver.com
이재훈 학부 choonie93@naver.com
엄준용 학부 eum1230@naver.com
졸업생
Name Degree Present Status email
Abu Mahammed Taufiqual Islam M.S.(2015) Ph.D.course at University of Melbourne, Australia ataufiqual@student.unimelb.edu.au
제브라피쉬 이미징
사진 갤러리
논문
  • Kim, M., Cho, H., Kwon, G., Kang, Y., Kwon, S.,Her, S., Kee, Y., Park, J.H.Y. (2015) Benzyl isothiocyanate suppresses high-fat diet-stimulated mammary tumor progression via the alteration of tumor microenvironments in obesity-resistant BALB/c mice. Mol Carcinogen, 54(1):72-82
  • Kwon, S., Park, O.K., Nie, S., Kwak, J., Hwang, B.J., Bronner, M.E. and Kee, Y. (2014) Bioinformatic analysis of nematode migration-associated genes identifies novel vertebrate neural crest markers. PLOS One, 9(7), e103024
  • Islam A.M.T., Kwak, J., Jung, Y.J., and Kee, Y. (2014) Animal models of Amyotrophic lateral sclerosis and Huntington's disease. Genes Genomics 36, 399-413
  • Raslan, A.A. and Kee, Y. (2013) Tackling neurodegenerative disease: animal models of Alzheimer's disease and Parkinson's disease. Genes Genomics 35, 425-40
  • Kee, Y. and Kim, N. (2013) Meeting report: Genetics and genome engineering. Genes Genomics 35, 411-3
  • Kim, M.J., Park, S.K., Choi, V.N., Seo,S., Lee, S., Kee, Y. and Hwang, B. (2013) Enzymatic production and expression of shRNAmir30 from cDNAs. Genes Genomics 35, 395-403
  • Kwak,J., Park,O.K., Jung, Y.J., Hwang, B., Kwon, S. and Kee, Y. (2013) Live image profiling of neural crest lineages in zebrafish transgenic lines. Mol Cells 35, 255-60
  • Nie, S., Kee, Y. and Bronner-Fraser, M. (2011) Caldesmon regulates actin dynamics to influence cranial neural crest migration in Xenopus. Mol Biol Cell 22, 3355-65
  • Nie, S., Kee, Y. and Bronner-Fraser, M. (2009) Myosin X is critical for the migratory ability of Xenopus cranial neural crest cells. Dev Biol 335, 132-42
  • Demyanenko, A.V., Zhao, L., Kee, Y., Nie, S., Fraser, S.E. and Tyszka, J.M. (2009) A uniplanar three-axis gradient set for in vivo magnetic resonance microscopy. J Mag Res 200, 38-48
  • Kee, Y., Hwang, B., Sternberg, P.W. and Bronner-Fraser, M. (2007) Evolutionary conservation of cell migration genes: from nematode neurons to vertebrate neural crest. Genes Dev 21, 391-6.
  • Hoeling, B.M., Feldman, S.S., Strenge, D.T., Bernard, A., Hogan, E.R., Petersen, D.C., Fraser, S.E., Kee, Y., Tyszka, J.M., and Haskell, R.C. (2007) A Motion-sensitive 3D optical coherence microscope operating at 1300 nm for the visualization of early frog development. Proc of SPIE 6429, 64292T-1-9.
  • Kee, Y. and Bronner-Fraser, M. (2005) To proliferate or to die: role of Id3 in cell cycle progression and survival of neural crest progenitors. Genes Dev 19, 744-55.
  • Kee, Y. and Bronner-Fraser, M. (2001) The transcriptional regulator Id3 is expressed in cranial sensory placodes during early avian embryonic development. Mech Dev109, 337-340.
  • Kee, Y. and Bronner-Fraser, M. (2001) Temporally and spatially restricted expression of the helix-loop-helix transcriptional regulator Id1 during avian embryogenesis. Mech Dev 109, 331-335.
  • Kee, Y. and Bronner-Fraser, M. (2001) Id4 expression and its relationship to other Id genes during avian embryonic development. Mech Dev 109, 341-345.
  • Hazuka C.D., Foletti D.L., Hsu S.C., Kee Y., Hopf F.W., and Scheller R.H. (1999) The sec6/8 complex is located at neurite outgrowth and axonal synapse-assembly domains. J. Neurosci19, 1324-1334.
  • Kee Y.,Yoo J.S., Hazuka C.D., Peterson K.E., Hsu S.C., and Scheller R.H. (1997) Subunit structure of the mammalian exocyst complex. Proc. Natl. Acad. Sci. USA 94, 14438-14443.
  • Hsu S.C., Ting A.E., Hazuka C.D., Davanger S., Kenny J.W., Kee Y., and Scheller R.H. (1997) The mammalian brain rsec6/8 complex. Neuron 17, 1209-1219.
  • Kee Y. and Scheller R.H. (1996) Localization of synaptotagmin-binding domains on syntaxin. J. Neurosci 16, 1975-1981.
  • Kee Y., Lin R.C., Hsu S.C., and Scheller R.H. (1995) Distinct domains of syntaxin are required for synaptic vesicle fusion complex formation and dissociation. Neuron 14, 991-998.
  • Olney R.C., Smith R.L., Kee Y., and Wilson D.M. (1993) Production and hormonal regulation of insulin-like growth factor binding proteins in bovine chondrocytes. Endocrinology 133, 563-570.
  • Nelson S.F., McCusker J.H., Sander M.A., Kee Y., Modrich P., and Brown P.O. (1993) Genomic mismatch scanning: a new approach to genetic linkage mapping. Nature Genetics 4, 11-18.
  • Kee Y., Jung E.M., Seol J.H., Lee C.H., Ha D.B., and Chung C.H. (1990) Turnover of glycerol kinase during starvation in Escherichia coli. Mol Cells 1, 99-102.
  • Kee Y., Park S.C., Ha D.B., and Chung C.H. (1989) Correlation between malignant phenotypes and changes in overall proteolytic capacity of human cervix and liver cancer. Korean J. Zool. 32, 48-54.
  • Kee Y., Park S.C., Ha D.B., and Chung C.H. (1988) Relation of the activities of plasminogen activator and plasmin-like protease with malignant behavior of skin tumor of rats. Korean J. Zool. 31, 185-190.
  • Kee Y., Lee Y.S., Goldberg A.L., and Chung C.H. (1988). Improved methods for purification and assay of glycerol kinase from Escherichia coli. J. Chromatography (Biomedical Applications) 428, 345-351.
특허
  • Zebrafish behavior pattern test device for drug screen. Korea patent number 10-1476355 (December 18, 2014), Korea patent application number 10-2014-0007668 (January 22, 2014). Inventors: Yun Kee; Byung Joon Hwang; Noo Li Jeon; Jina Kwak
  • Magnetic resonance stage microscope. US patent number 7633295 (Issued at December 15, 2009). Inventors: Julian M. Tyszka; Andrey V. Demyanenko; Yun Kee; Scott E. Fraser
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